'Plant Breeding: Sustaining the Future' Abstracts of the XVIth EUCARPIA Congress, Edinburgh, Scotland, 10-14 September 2001 BARLEY ESTS: A RESOURCE FOR NOVEL MARKERS R. KOTA, M. WOLF, T. THIEL, W. MICHALEK, A. GRANER Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstr. 3, 06466 Gatersleben, Germany

With the development of EST sequencing projects, a wealth of sequence information is being generated allowing to genetically map these ESTs.  In order to initiate a systematic approach to develop an EST-map of barley (Hordeum vulgare L.), we have developed RFLP, SSR and SNP markers. RFLPs are being identified and mapped using conventional techniques while a set of SSRs was identified by mining the EST-database for corresponding motifs. SNPs were identified between the parental genotypes of our three mapping populations by comparative sequencing. Once a SNP has been established, it is mapped by denaturing high-performance liquid chromatography (DHPLC). Employing these three assays, we plan to place over 1000 genes onto a consensus EST map of barley with preliminary results being presented.