'Plant Breeding: Sustaining the Future' Abstracts of the XVIth EUCARPIA Congress, Edinburgh, Scotland, 10-14 September 2001 IDENTIFICATION OF MOLECULAR MARKERS CLOSELY LINKED TO YIELD IN TWO MAIZE POPULATIONS D. IGNJATOVIĆ-MICIĆ, T. ĆORIĆ, D. KOVAČEVIĆ, V. LAZIĆ-JANČIĆ, M. IVANOVIĆ, S.A. QUARRIE Maize Research Institute, S. Bajica1, 11 080 Belgrade-Zemun, Yugoslavia

This study was conducted to identify chromosome regions involved in grain yield expression in maize. F₂ plants of two crosses, B73 x Mo17 and L1 x Mo17, were selfed and also top-crossed to F7R as tester. Testcrosses were evaluated for grain yield. Based on yield data F₃ families with the highest and lowest test cross yields were selected for bulk segregant analysis (BSA). BSA of high and low yield bulks was done with 60 RFLP probes from the UMC core set in combination with three restriction enzymes - EcoRI, BamHI and Hind III. Differences in RFLP allele frequencies of high and low yield bulks identified regions at chromosomes 1, 2, 6 and 10 involved in grain yield expression in one or both analysed maize test cross populations.