'Plant Breeding: Sustaining the Future' Abstracts of the XVIth EUCARPIA Congress, Edinburgh, Scotland, 10-14 September 2001 MITOGENIC EFFECTS OF PHYTOHEMAGGLUTININ (PHA) ON ISOLATED MICROSPORE CULTURE OF BARLEY  M. KHOSROWCHAHLI, M.H. NAGHILOU, M. MOGHADDAM  Department of Biology, University of Tabriz, Tabriz, Iran

Mitogenic effects of phytohemagglutinin, form "M", on isolated microspore culture of barley was studied. The L1 modified medium with 3 mg/l phenylacetic acid and 1 mg/l benzylaminopurin supplemented with 0.3 and 0.7 mg/l PHAm were used. The number of embryos, number of green plantlets and number of albino plantlets per 40000 microspores for this medium was not significantly different from the control. However, a decrease of the period of time for embryo development from 20-24 days to 18-20 days was observed, which can be attributed to the mitogenic effects of PHA.