'Plant Breeding: Sustaining the Future' Abstracts of the XVIth EUCARPIA Congress, Edinburgh, Scotland, 10-14 September 2001 LINKAGE OF MICROSATELLITE MARKERS TO AN ALUMINIUM TOLERANCE GENE IN WHEAT P. CZEMBOR, M. WIEWIÓRA, H.J. CZEMBOR Plant Breeding and Acclimatization Institute, Radzikow, 05-870 Blonie, Poland

An attempt was made to identify microsatellite markers linked to gene(s) responsible for tolerance reaction to aluminium toxicity in spring wheat. For the purpose of the bulked segregant analysis (BSA) one tolerant pool was established (5 tolerant individuals from F₃ families) and sensitive one (5 sensitive individuals from F₃ families) together with sensitive parent HANNO and tolerant parent BH 1146. This material was screened with 54 sets of primers which amplify in PCR (Polymerase Chain Reaction) microsatellite loci on 2D, 3D, 4D and 5A chromosomes where aluminium tolerance genes were identified. Two markers Xgwm165 and Xgdm125 proved closer linkage to the earlier identified gene Alt_BH on 4DL chromosome and were mapped in the distance of 8,6 cM and 3,2 cM respectively.