'Plant Breeding: Sustaining the Future' Abstracts of the XVIth EUCARPIA Congress, Edinburgh, Scotland, 10-14 September 2001 IDENTIFICATION OF WHEAT-BARLEY TRANSLOCATIONS USING MOLECULAR GENETIC AND CYTOGENETC METHODS M. MOLNÁR-LÁNG, G. LINC, E. D’NAGY Agricultural Research Institute of the Hungarian Academy of Sciences, 2462 Martonvásár, P.O.Box. 19, Hungary

Wheat-barley translocations were detected using genomic in situ hybridization (GISH) in backcross progenies originating from hybrids (Chinese Spring × Betzes, Martonvásári 9 kr1 × Igri)multiplied in tissue culture. Homozygous translocations were selected from the selfed progenies. Identification of the translocations have beeen started using molecular genetic and cytogenetic methods. The detection of the translocated chromosomes was carried out using GISH, after rinsing off the preparations, fluorescent in situ hybridization (FISH) was carried out with two repetitive probes (pAs1, pSc119) on the same slides. The wheat chromosome segments in some translocated chromosomes could be identified with the help of the two repetitive probes. Small differences were found in the hybridization pattern of the wheat line Martonvásári 9 kr1 compared to that of the wheat variety Chinese Spring. The identification of the barley chromosome segments has begun using barley microsatellite markers.