'Plant Breeding: Sustaining the Future' Abstracts of the XVIth EUCARPIA Congress, Edinburgh, Scotland, 10-14 September 2001 MAPPING WHEAT ESTS BY HAPLOTYPE SPECIFIC SNPS D.J. SOMERS, R. KIRKPATRICK, M. MONIWA, A. WALSH, J.P. GUSTAFSON Agriculture and Agri-Food Canada, Cereal Research Centre, 195 Dafoe Rd, Winnipeg, R3T 2M9, MB Canada

The abundance of EST sequence information for wheat available now, makes it possible to develop PCR primers capable of amplifying haplotype specific sequences in wheat.  The haplotype sequences were identified by cluster analysis of an unassembled, EST database (60,000 sequences).  The analysis consisted of 1) physical mapping with the Chinese Spring nulli/tetrasonic lines and a Chinese Spring ditelomeric deletion series and 2) genetic mapping including four polymorphic crosses.  Overall, 63% of the haplotype EST sequences could be physically mapped and 11% could be genetically mapped.  This will facilitate a good comparison of physical and genetic mapping of ESTs in wheat and a study allelic variation.  The haplotype specific SNPs were also capable of being used for wheat variety identification.